专利名称：METHODS FOR INCREASING THE
EFFICIENCY OF RECOMBINANT AAVPRODUCT
发明人：SAMULSKI, Richard, J.,XIAO, Xiao,SNYDER,
Richard
申请号：EP98915603.9申请日：19980414公开号：EP1007637B1公开日：20040630
摘要：The present invention relates to methods and compositions for increasing theproduction of high titre stocks of recombinant AAV (rAAV) through regulation ofexpression of the AAV REP and CAP proteins. The methods and compositions of theinvention are based on the observation that the low level expression of the AAV REPprotein increases the production of AAV viral capsid protein and efficiency of packagingresulting in production of higher titre recombinant viral stocks. The invention
encompasses recombinant AAV vectors that direct the expression of AAV REP and CAPproteins (see the figure) and the use of such vectors for the production of novel stablecell lines capable of generating high titre rAAV vectors. The invention provides methodsfor regulating the expression of the AAV REP gene at the transcriptional and post-translational level.
申请人：CELL GENESYS INC,UNIV NORTH CAROLINA
地址：US,US
国籍：US,US
代理机构：Schüssler, Andrea, Dr.
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