METHOD FOR DETECTING MICROORGANISMS IN A SAMPLE

专利名称：METHOD FOR DETECTING

MICROORGANISMS IN A SAMPLE

发明人：SNAIDR, JIRI申请号：EP0003989申请日：20000504公开号：WO0068421A3公开日：20010809

摘要：The invention relates to a method for detecting microorganisms in a sample bymeans of a nucleic acid probe. Conventional detection methods are, for example, the in-situ hybridization of microorganisms with fluorescence-labeled oligonucleotide probes(fluorescent in-situ hybridization). A disadvantage of said method is that an

epifluorescence microscope is required for evaluating the results. According to theinvention, the disadvantages of the in-situ hybridization method are overcome byhybridizing the microorganisms to be detected in a sample with a specific nucleic acidprobe, removing non-hybridized nucleic acid probe molecules, separating and thendetecting and optionally quantifying the hybridized nucleic acid probe molecules.

申请人：VERMICON AG,SNAIDR, JIRI

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