专利名称：RNA sequence-specific mediators of RNA
interference
发明人：Thomas Tuschl,Phillip D. Zamore,Phillip A.
Sharp,David P. Bartel
申请号：US13008636申请日：20110118公开号：US08957197B2公开日：20150217
摘要：The present invention relates to a in vitro system which was used to
demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length.Furthermore, when these 21-23 nt fragments are purified and added back to extracts,they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 ntfragments are the sequence-specific mediators of RNA degradation. A molecular signal,which may be their specific length, must be present in these 21-23 nt fragments to recruitcellular factors involved in RNAi. This present invention encompasses these 21-23 ntfragments and their use for specifically inactivating gene function. The use of thesefragments (or chemically synthesized oligonucleotides of the same or similar nature)enables the targeting of specific mRNAs for degradation in mammalian cells, where theuse of long dsRNAs to elicit RNAi is usually not practical, presumably because of thedeleterious effects of the interferon response. This specific targeting of a particular genefunction is useful in functional genomic and therapeutic applications.
申请人：Thomas Tuschl,Phillip D. Zamore,Phillip A. Sharp,David P. Bartel
地址：Goettingen DE,Northborough MA US,Newton MA US,Brookline MA US
国籍：DE,US,US,US
代理机构：Lando & Anastasi, LLP
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